abstract：BACKGROUND:We tested the hypothesis that the best time for genetic modification is while the cell viability of the graft is reduced for long-term preservation. The hemagglutinating virus of Japan (HVJ)-liposome method, a nonviral gene transfer technique, was used with a luciferase gene to test the efficacy of protein i...

journal_title：Transplant immunology

authors： Kita J,Kobayashi E,Hishinuma A,Kaneda Y

更新日期：2003-01-01 00:00:00

abstract：:Adenovirus-mediated CTLA4Ig gene transfer has been reported to enhance graft survival in several rodent transplantation models. In this study, we investigated the efficacy of ex vivo and systemic transfer of the CTLA4Ig gene by adenoviral vectors in pancreatic islet allo-transplantation. Islet grafts from BN rats were...

journal_title：Transplant immunology

authors： Akamaru Y,Ito T,Uchikoshi F,Maeda A,Tori M,Kiyomoto T,Komoda H,Miao G,Matsuda H

更新日期：2003-01-01 00:00:00

abstract：:We investigated the dynamics of the CD4+ and CD8+ lymphocyte subsets, and the expression of activation markers in cardiac transplant recipients. We tested 132 peripheral blood samples from 62 cardiac transplant recipients using fluorescent staining and flow cytometry analysis. The results were correlated with histolog...

journal_title：Transplant immunology

authors： Creemers P,Brink J,Wainwright H,Moore K,Shephard E,Kahn D

更新日期：2002-11-01 00:00:00

abstract：:The immune system is an adaptive defense system capable of specifically recognizing and eliminating an apparently limitless variety of foreign invaders. This highly specific response is provided by T and B lymphocytes. The enormous diversity of the antigen-specific receptors on these cells is generated by random rearr...

journal_title：Transplant immunology

authors： Arnold B

更新日期：2002-08-01 00:00:00

abstract：:Administration of anti-D immunoglobulin to D- women after delivery of a D+ infant has dramatically reduced the number of immunised women and cases of haemolytic disease of the fetus and newborn. The use of monoclonal anti-D might alleviate some of the pressures on maintaining adequate supplies of plasma sourced anti-D...

journal_title：Transplant immunology

authors： Kumpel BM

更新日期：2002-08-01 00:00:00

abstract：:Naturally occurring antibodies to the galactose alpha1,3 galactose (alpha gal) epitope expressed on xenogeneic grafts are a major barrier to organ transplantation in humans. Porcine small intestinal submucosa (SIS) expresses the alpha gal epitope and is currently being used as a bioscaffold for tissue remodeling. To e...

journal_title：Transplant immunology

authors： Raeder RH,Badylak SF,Sheehan C,Kallakury B,Metzger DW

更新日期：2002-06-01 00:00:00

abstract：:HMG-CoA reductase inhibitors, or statins, are effective lipid lowering agents, extensively used in medical practice. Statins have never been shown to be involved in the immune response, although few clinical reports have suggested a better outcome of cardiac transplantation in patients under pravastatin therapy. Major...

journal_title：Transplant immunology

authors： Mach F

更新日期：2002-05-01 00:00:00

abstract：:Transplant rejection is a multifactorial process involving complex interactions between components of the innate and the acquired immune system. In view of the shortage of donor organs available for transplantation, xenotransplantation of pig organs into man has been considered as a potential solution. However, in com...

journal_title：Transplant immunology

authors： Roos A,Daha MR

更新日期：2002-05-01 00:00:00

abstract：:Using specific cell surface receptors lymphocytes continuously sample their environment. Maturation of the immune system and initiation of a specific immune response rely on an array of extracellular cues that elicit complex intracellular biochemical signals. Essential molecules involved in signal transduction from im...

journal_title：Transplant immunology

authors： Kiefer F,Vogel WF,Arnold R

更新日期：2002-05-01 00:00:00

abstract：:Pancreas grafts do not survive for lengthy periods, especially in a high-responder rat combination. Recent data indicated that a combined spleen/pancreas graft protects against acute graft rejection and induces donor-specific tolerance. In this study, we performed a combination spleen/pancreas transplantation using hi...

journal_title：Transplant immunology

authors： Sakuma Y,Uchida H,Nagai H,Kobayashi E

更新日期：2001-10-01 00:00:00

abstract：OBJECTIVE:The immunomodulator, FTY720, lowers the peripheral lymphocyte count (PLC) by inducing migration of circulating lymphocytes to secondary lymphoid organs. We investigated the efficacy of mono- vs. combined-FTY720/CsA therapy on graft survival (GS) and on lowering the PLC in a solid organ and a skin graft model,...

journal_title：Transplant immunology

authors： Nikolova Z,Hof A,Baumlin Y,Hof RP

更新日期：2001-02-01 00:00:00

abstract：:Upon activation, T cells express CD40L, a member of the TNF cytokine superfamily, which serves as a ligand for CD40 on antigen presenting cells, including dendritic cells, B cells, and macrophages. While initial studies on the function of CD40 focused its role in the regulation of B cell activation, more recent studie...

journal_title：Transplant immunology

authors： Bingaman AW,Pearson TC,Larsen CP

更新日期：2000-11-01 00:00:00

abstract：:Calcineurin a calmodulin-dependent phosphatase plays a critical role in calcium-dependent activation of T-lymphocytes and is the major target for the inhibitory actions of the immunosuppressive drugs Tacrolimus (FK506) and Cyclosporin A (CsA). Calcineurin is a dimeric protein consisting of distinct A (catalytic) and B...

journal_title：Transplant immunology

authors： Brogan IJ,Pravica V,Hutchinson IV

更新日期：2000-06-01 00:00:00

abstract：:Dendritic cells (DC) are considered to be the major cell type responsible for induction of primary immune responses. While they have been shown to play a critical role in eliciting allosensitization via the direct pathway, there is evidence that maturational and/or activational heterogeneity between DC in different do...

journal_title：Transplant immunology

authors： Steptoe RJ,Patel RK,Subbotin VM,Thomson AW

更新日期：2000-03-01 00:00:00

abstract：:Today, most clinically used methods for analysis of alloreactivity in organ transplantation are based on humoral immunity. In order to study the cellular alloresponse, a rat kidney transplantation model with culturing of graft infiltrating lymphocytes was developed. Kidney transplantations between inbred rat strains w...

journal_title：Transplant immunology

authors： Engstrand M,Johnsson C,Korsgren O,Tufveson G

更新日期：1999-12-01 00:00:00

abstract：:The novel immunosuppressive agents malononitrilamides (MNA) 279 and 715 are derivatives of A77 1726, the primary metabolite of leflunomide. The effects of these agents have been previously demonstrated in rat skin and cardiac allo- and xenotransplant models. The aim of this study was to evaluate the combination of MNA...

journal_title：Transplant immunology

authors： Qi Z,Simanaitis M,Ekberg H

更新日期：1999-09-01 00:00:00

abstract：:The interaction of antibodies with cell surface antigens may induce redistribution of immune complexes, followed by antigen depletion, with increased resistance to injurious effect of antibody and complement (antigenic modulation). Human natural antibodies to Gal alpha 1,3Gal beta 1,4GlcNAc-R (alpha Gal) epitopes expr...

journal_title：Transplant immunology

authors： Maruyama S,Cantu E3rd,Pernis B,Galili U,Godman G,Stern DM,Andres G

更新日期：1999-06-01 00:00:00

abstract：:Administration of anti-intercellular adhesion molecule (ICAM)-1 monoclonal antibody (mAb) plus anti-lymphocyte function associated antigen (LFA)-1 mAb induces tolerance in murine cardiac transplantation, while anti-vascular cell adhesion molecule (VCAM)-1 mAb plus anti-very late antigen (VLA)-4 mAb administration prol...

journal_title：Transplant immunology

authors： Suzuki J,Isobe M,Izawa A,Takahashi W,Yamazaki S,Okubo Y,Amano J,Sekiguchi M

更新日期：1999-03-01 00:00:00

abstract：:Mixed lymphocyte reaction (MLR) assays were performed serially over 24 months in 19 first cadaver renal transplant recipients. Immunosuppression consisted of cyclosporine, methylprednisolone and azathioprine. Cyclosporine was withdrawn at 6 months postoperatively. The MLR reactivity gradually decreased over the first ...

journal_title：Transplant immunology

authors： Creemers P,Pascoe MD,Pontin AR,Kahn D

更新日期：1998-12-01 00:00:00

abstract：:Previously, we developed a well-tolerated single-day protocol for induction of stable multilineage chimerism and permanent donor-specific tolerance across major histocompatibility complex (MHC) barriers, with preservation of the host's normal immune responses. In our murine model, recipient mice were treated with a si...

journal_title：Transplant immunology

authors： de Vries-van der Zwan A,van der Pol MA,de Waal LP,Boog CJ

更新日期：1998-09-01 00:00:00

abstract：:The means by which methotrexate (MTX) mediates immunosuppression at low doses remains to be elucidated. MTX has been shown to inhibit the adherence of neutrophils and fibroblasts to endothelial cells in vitro. The hypothesis that MTX treatment may affect cellular adherence by downregulating cell adhesion molecule expr...

journal_title：Transplant immunology

authors： Ciesielski CJ,Pflug JJ,Mei J,Piccinini LA

更新日期：1998-06-01 00:00:00

abstract：:Mycophenolate mofetil (MMF) a potent immunosuppressive agent, has recently been approved for clinical use (CellCept) in renal transplant patients in combination with cyclosporine (CsA). With the expanded use of tacrolimus (Prograf) as well in renal transplant patients, there is a lack of pharmacokinetic studies clarif...

journal_title：Transplant immunology

authors： Zucker K,Rosen A,Tsaroucha A,de Faria L,Roth D,Ciancio G,Esquenazi V,Burke G,Tzakis A,Miller J

更新日期：1997-09-01 00:00:00

abstract：:Limiting dilution analysis technique was used to enumerate the circulating precursor frequency of donor and third-party-reactive helper T lymphocytes (HTLpf) in 28 renal allograft recipients before (pre-tx) and at three intervals (T1: 60-90 days, T2: 120-180 days, T3: 360-1620 days) after transplantation (post-tx). Tw...

journal_title：Transplant immunology

authors： Beik AI,Higgins RM,Lam FT,Morris AG

更新日期：1997-06-01 00:00:00

abstract：:The aetiologies of accelerated or transplant-associated coronary artery disease (TxCAD) following cardiac transplantation and chronic rejection following renal transplantation remain ill-defined. Previous studies have used Western blotting to demonstrate an association between the formation of anti-endothelial (anti-E...

journal_title：Transplant immunology

authors： Ferry BL,Welsh KI,Dunn MJ,Law D,Proctor J,Chapel H,Yacoub MH,Rose ML

更新日期：1997-03-01 00:00:00

abstract：:Previous work on the facilitation of xenogeneic and allogeneic bone marrow engraftment in irradiated mice and dogs with transferrins allowed the development of a model for induction of an apparently durable state of immunological unresponsiveness or 'tolerance' in chemically immunosuppressed mice. The system is based ...

journal_title：Transplant immunology

authors： Pierpaoli W,Lesnikov VA,Lesnikova MP,Arrighi S,Bardotti A

更新日期：1996-12-01 00:00:00

abstract：:We have developed a highly sensitive method for the simultaneous crossmatching of T and B cells using three-colour flow cytometry. This method identifies T and B cell populations in a lymphocyte sample and analyses each for the presence of bound patient IgG alloantibodies. A fluorescein isothiocyanate conjugated rabbi...

journal_title：Transplant immunology

authors： Robson A,Martin S

更新日期：1996-09-01 00:00:00

abstract：:Experimental data show that relatively low concentrations of 15-deoxyspergualin (DSG) inhibit the induction of cytotoxic T lymphocytes (CTL) and the generation of antibody-producing cells. Considerably higher concentrations of DSG are required to inhibit proliferative responses. In this in vitro study, the effects of ...

journal_title：Transplant immunology

authors： Borg AJ,Kumagai-Braesch M,Möller E

更新日期：1996-06-01 00:00:00

abstract：:Clinical ophthalmologists are highly successful in grafting allogeneic corneas onto healthy eye graft beds--only a small percentage are rejected. By contrast, a high percentage of allografts are rejected when grafted into vascularized or '"high-risk' recipient eyes. The following experiments test the hypothesis that d...

journal_title：Transplant immunology

authors： Ksander BR,Sano Y,Streilein JW

更新日期：1996-03-01 00:00:00

abstract：:Introduction of MHC class I Kb cDNA via recombinant retrovirus into B10.AKM (Kk) bone marrow cells (BMC) has been shown to confer specific hyporesponsiveness to B10.MBR (Kb) allogeneic skin grafts in lethally irradiated B10.AKM recipients of the transduced syngeneic BMC. We have recently developed a nonmyeloablative c...

journal_title：Transplant immunology

authors： Hayashi H,LeGuern C,Sachs DH,Sykes M

更新日期：1996-03-01 00:00:00

abstract：:Desferrioxamine (DFO), commonly used in therapy as a chelator of ferric ion in disorders of iron overload, is a potent inhibitor of human cytomegalovirus (HCMV) replication in cultured fibroblast cells. Moreover, DFO has immunomodulatory activity both in vitro and in vivo. We studied DFO effects on HCMV replication in...

journal_title：Transplant immunology

authors： Cinatl J,Scholz M,Weber B,Cinatl J,Rabenau H,Markus BH,Encke A,Doerr HW

更新日期：1995-12-01 00:00:00

abstract：:Mouse hearts transplanted into MHC disparate donors are usually rejected 1 week after placement. It is widely accepted that alloantigen-reactive helper T lymphocytes (HTL) and cytotoxic T lymphocytes (CTL) are the key mediators of acute allograft rejection. This report demonstrates that the presence or absence of 'tra...

journal_title：Transplant immunology

authors： Bishop DK,DeBruyne LA,Chan S,Xu S,Eichwald EJ

更新日期：1995-09-01 00:00:00

abstract：:Circulating host lymphocytes recognize, bind to, become activated by, and infiltrate engrafted allogeneic tissues. The mechanisms responsible for these early events which lead to acute immunological rejection have not been precisely defined. We have examined sequentially in vitro lymphocyte binding patterns in a kidne...

journal_title：Transplant immunology

authors： Tullius SG,Heemann UW,Zeilinger K,Azuma H,Tamatami T,Miyasaka M,Tilney NL

更新日期：1995-06-01 00:00:00

abstract：:C3H mice were immunized by repeated skin grafting with B10.BR tail skin. Ten days after the last immunization mice received 100 micrograms (intravenously) of a variety of different monoclonal antibodies (mAbs: anti-ICAM-1, -LFA-1, -VCAM-1, -VLA-4), alone or in combination, followed by further B10.BR skin grafts. Contr...

journal_title：Transplant immunology

authors： Gorcyznski RM,Chung S,Fu XM,Levy G,Sullivan B,Chen Z

更新日期：1995-03-01 00:00:00

abstract：:A rapid and robust limiting dilution assay was developed to measure the frequency of donor-reactive, IL-2 (interleukin 2) producing, helper T lymphocytes in the peripheral T cell population of organ allograft recipients. The IL-2 bioassay was performed using two methodologies to assess the response of CTLL-2 indicator...

journal_title：Transplant immunology

authors： Cattell EL,Cunningham AC,Bal W,Taylor RM,Dark JH,Kirby JA

更新日期：1994-12-01 00:00:00

abstract：:TCR-beta (T cell receptor-beta chain) transgenic mice have altered lymphocyte development. TCR-beta transgenic mice are hyporesponsive to alloantigens in vivo and are deficient in gamma delta T cells. In order to begin a study of the relationship between a deficiency of alloreactive gamma delta cells and the defective...

journal_title：Transplant immunology

authors： Rimm IJ,Fruman DA,Abhyankar S,Sakamoto H,Orme IM,Milstone D,Seidman JG,Ferrara JL

更新日期：1994-09-01 00:00:00

abstract：:Over the last 25 years the importance of the humoral presensitization status of transplant recipients has increasingly been recognized. With greater understanding of the precise specificity of the pretransplant antibody status of recipients it is clear that the graft survival figures will improve. The development of n...

journal_title：Transplant immunology

authors： Shenton BK

更新日期：1994-06-01 00:00:00

abstract：:Tissue eosinophils have been previously implicated in allograft rejection and graft loss. The aim of this study was to evaluate the role of eosinophils in acute renal allograft rejection. Data from 71 patients with 114 renal biopsies with acute allograft rejection were compared with those from 26 controls. The median ...

journal_title：Transplant immunology

authors： Hongwei W,Nanra RS,Stein A,Avis L,Price A,Hibberd AD

更新日期：1994-01-01 00:00:00

abstract：:Human anti-pig antibodies were obtained by perfusing pig hearts (n = 4) and kidneys (n = 8) with human AB or O plasma followed by elution with 3 M NaSCN. The antibodies were screened against a panel of 132 synthetic carbohydrates conjugated to bovine serum albumin using an enzyme-linked immunoassay. An anti-immunoglob...

journal_title：Transplant immunology

authors： Cooper DK,Good AH,Koren E,Oriol R,Malcolm AJ,Ippolito RM,Neethling FA,Ye Y,Romano E,Zuhdi N

更新日期：1993-01-01 00:00:00

abstract：:Keratinocytes are activated to express MHC class II and ICAM-1 molecules during cutaneous inflammatory reactions. It is controversial how the interaction between these 'nonprofessional' antigen presenting cells (APC) and infiltrating T cells affects the local inflammatory response. To address this issue we analyzed wh...

journal_title：Transplant immunology

authors： de Bueger M,Bakker A,Goulmy E

更新日期：1993-01-01 00:00:00

abstract：:Alloreactivity probably involves T cell recognition of peptides bound to allogeneic HLA molecules. In order to study whether T cells recognizing common viral antigens presented by self HLA molecules also recognize some allogeneic HLA molecules we generated T lymphocyte clones (TLC) specific for herpes simplex virus (H...

journal_title：Transplant immunology

authors： Gjertsen HA,Lundin KE,Hansen T,Thorsby E

更新日期：1993-01-01 00:00:00